A genomic integration method to visualize localization of endogenous mRNAs in living yeast

Liora Haim; Gadi Zipor; Stella Aronov; Jeffrey Gerst

doi:10.1038/NMETH1040

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A genomic integration method to visualize localization of endogenous mRNAs in living yeast

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A genomic integration method to visualize localization of endogenous mRNAs in living yeast

Liora Haim, Gadi Zipor, Stella Aronov and Jeffrey Gerst

Nature Methods, Vol.4(5), pp.409-412

May/2007

DOI: https://doi.org/10.1038/NMETH1040

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Abstract

mRNA localization may be an important determinant for protein localization. We describe a simple PCR-based genomic-tagging strategy (m-TAG) that uses homologous recombination to insert binding sites for the RNA-binding MS2 coat protein (MS2-CP) between the coding region and 3' untranslated region (UTR) of any yeast gene. Upon coexpression of MS2-CP fused with GFP, we demonstrate the localization of endogenous mRNAs (ASH1, SRO7, PEX3 and OXA1) in living yeast (Saccharomyces cerevisiae).

Details

Title: A genomic integration method to visualize localization of endogenous mRNAs in living yeast
Creators: Liora Haim (null) - 972WIS_INST___111
Gadi Zipor (null)
Stella Aronov (null)
Jeffrey Gerst (null) - 972WIS_INST___111
Resource Type: Journal article
Publication Details: Nature Methods, Vol.4(5), pp.409-412; May/2007
Number of pages: 4
Language: English
DOI: https://doi.org/10.1038/NMETH1040
Record Identifier: 993263512403596

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